Blood Bank Case Study

------------------------------------------------- Analytical Toxicology Background In analytical toxicology especially to give out doses, several(prenominal) methods can be used. The methods can be categorized deal on their principle of assay which are chemical, spectrometric, chromatographic, electrophoretic and immunochemical assays. each principle of analysis consists of round proficiencys that can be used. However, immunoassay is normally used in drug viewing overdue to sensitivity, specificity and low bell than other methods. The half-life of drug excessively used in toxicology analysis to helps in remedial observe and accessing drug abuse. Introduction Immunoassays are bioanalytical method in which the quantitation of analyte depends on the reply of antigen (analyte) and antibody. (Ibrahim A. Darwish , 2006). Immunoassay principle is based on competitive cover charge which specific antibody is used to chance on the presence of analyte or its metabolite in the sample tested. This method consists of some techniques which include: I. Radioimmunoassay (RIA) II. Enzyme immunoassay (EIA) III. Enzyme figure immunoassay technique (EMIT) IV. Enyzme cerebrate immunoasorbent assay (ELISA) V. Fluorescence polarization immunoassay (FPIA) VI. Cloned enzyme donor immunoassay (CEDIA) I.

Radioimmunoassay (RIA) This technique is no longer commonly used due to foul up disposal issues where high cost of handling and disposal cost. The analyte in the sample is detected victimization radioisotope. In RIA, sample is coalesce with specific antibody to the tar ask analyte and tagged analyte w! ith radioactive iodine. Analyte in the sample compete with labeled analyte for antibody. da Gamma reproduction is used to measure the labeled analyte bind with antibody per slender (CPM). II. Enzyme immunoassay (EIA) EIA is similar to RIA but instead of measuring radiation syndrome this technique measure enzyme activity. It consist of signal detection step which the firmness of purpose is measured optically by fluorescence...If you want to get a full essay, order it on our website: OrderCustomPaper.com

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